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01 ANTIOXIDANTS AND ANTIFIBROTICS PROPERTIES OF N-ACETYLCYSTEINE IN THE EXPERIMENTAL LIVER DAMAGE REVERTION GALICIA-MORENO M,* MONROY-RAMÍREZ HC,* ARAUZ J, * MURIEL P** *DEPARTAMENTO DE FARMACOLOGÍA, FACULTAD DE MEDICINA MEXICALI, U.A.B.C., MEXICALI, B.C. MÉXICO. **DEPARTAMENTO DE FARMACOLOGÍA, CINVESTAV-IPN, CIUDAD DE MÉXICO, MÉXICO. Background. N-acetylcysteine (NAC) is an antioxidant, precursor of reduced glutathione and inhibitor of different profibrotic cytokines involved in liver damage. The aim of this work was to evaluate the effect of NAC to reverse liver cirrhosis induced with carbon tetrachloride (CCl₄) in rats. Material and methods. Four groups of rats (n = 8) were performed. Group 1, control animals received the vehicle (oil). Group 2 was administered with intraperitoneal CCl4 (0.4 g/kg). Group 3 received CCl₄ and then NAC (300 mg/kg, orally). Group 4 received mineral oil (0.25 mL), and then NAC. CCl₄ and oil were administered by 2 months, three times per week and NAC and CMC only by 1 month daily after treatment with CCl₄. Alanine aminotransferase (ALT) was measured in plasma. Collagen, glycogen and MDA levels and reduced glutathione were esteemed in liver samples; a histopathologycal analysis was performed. Results. ALT enzyme activity increased significantly after 2 months of CCl₄-administration. Discontinuation of CCl₄ for 1 month resulted in a decrease levels of ALT enzyme activity to normal values. MDA levels increased while GSH/GSSG ratio in liver decreased by the administration of CCl₄ for 2 months; discontinuation of CCl₄ did not lead to normal values of these parameters, again NAC prevented both effects. Liver glycogen content decreased by CCl₄ intoxication, NAC restored normal glycogen levels. Fibrosis was quantified by measuring collagen levels, CCl₄-intoxication during 2 months produced an increased in liver collagen content. Discontinuation of CCl₄ did not lead to fibrolysis, NAC administration resulted in a partial but significant reversion of CCl₄-induced fibrosis. Conclusions. Our results strongly suggest that NAC was highly effective in reversing liver damage. This effect perhaps is related to the ability of NAC as scavenger as well as its antifibrogenic capacity. We are currently studying the expression of several cytokines related to liver damage as TGF-β, among others to be able to consider that NAC can be tested in fibrotic or cirrhotic patients under control trials. This work was supported by Beca PRODEP- apoyo a la incorporación de nuevos PTC UABC7PTC 570. Para mayor información, favor de descargar el archivo PDF correspondiente.

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2017-12-14   |   274 visitas   |   Evalua este artículo 0 valoraciones

Vol. 16 Núm.4. Julio-Agosto 2017 Pags. 645-692 Ann Hepatol 2017; 16(4)