Clasificación filogenética de Escherichia coli uropatógena y respuesta inmunometabólica en adultos mayores con infección urinaria en casas de reposo

Autores: Gonzales Rodriguez Arturo Octavio , Barrón Pastor Heli Jaime, Gutiérrez Villafuerte Cesar Arturo, Mitma de Barrón Yesica Llimpe, Huerta Canales Doris Virginia, Suarez Cunza Silvia

Resumen

Objectives: to characterize the immunometabolic response in the urine of elderly patients infected with uropathogenic Escherichia coli resident in elderly homes and determine its phylogenetic frequency. Materials and methods: descriptive and observational study in which 24 patients with urinary tract infection and 17 patients without urinary tract infection were enrolled. Were quantified Iron and TNF – ? in urine by spectrophotometric methods, the antioxidant capacity by ABTS + * assay and the phylogenetic determination of E. coli by the Clermont method. Results: the concentration of iron, TNF - ? and the antioxidant capacity in urine in patients with urinary tract infection were 175.40 ?g / L, 70.11 pg / mL and 1229.75 ?g / mL eq-VitC, while in patients without infection the values were of 67.4 ?g / L (p = 0.001), 87.16 pg / mL (p = 0.08) and 1069.14 ?g / mL eq-VitC (p = 0.61) respectively. In addition, 11 E. coli belonging to group B2 and 13 grouped in non - B2 (A, B1, D and E) were found. Conclusions: high availability of iron in the urine of elderly patients with urinary tract infection is evidenced, which stimulates bacterial proliferation. There is a high antioxidant capacity in the urine of patients with urinary tract infection and a greater clonal presence of non-B2 type E.coli in the urine of patients residing in elderly homes.

Palabras clave: biomarcadores adulto mayor filogenia Escherichia coli

2019-11-08   |   281 visitas   |   Evalua este artículo 0 valoraciones

Vol. 19 Núm.2. Julio-Diciembre 2019 Pags. 238-246 Arch Med Manizales 2019; 19(2)