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TRANSPLANT/LIVER SURGERY USE OF A COLLAGEN MATRIX AS A SCAFFOLD FOR THE HEPATIC TISSUE C ACEVEDO,* M MUÑOZ-VEGA,* B LEÓN,** MC GARCÍA DE LEÓN,* C PIÑA,** G GUTIÉRREZ-REYES,* D KERSHENOBICH* *HIPAM, UNIDAD DE MEDICINA EXPERIMENTAL, FACULTAD DE MEDICINA, UNAM/HOSPITAL GENERAL DE MÉXICO. MÉXICO, D.F., MÉXICO. **INSTITUTO DE INVESTIGACIONES EN MATERIALES, UNAM. MÉXICO, D.F., MÉXICO. INTRODUCTION AND OBJECTIVES: Recently, biomedicine has incorporated the use of biomaterials as scaffolds for the functional and structural recovery of damaged tissues. These kinds of materials are amply used in orthopedics, maxillofacial surgery and dentistry. Most of these materials favor cellular induction and conduction in the tissue, and enable cell proliferation in the tissue where they have been grafted. The Institute of Research on Materials from the National Autonomous University of Mexico have developed a novel collagen based matrix that has shown mechanical and compositional properties that would allow it to become a scaffold for different tissues. This material has been successfully used in the urinary tract; however it has not been use elsewhere. The purpose of our work was to test the biocompatibility of this novel collagen matrix in the fibrotic hepatic tissue and its possible use as a hepatic scaffold. MATERIAL AND METHODS: Male Wistar rats weighing 240 ± 20 g were administered CCl4 (33% v/v in olive oil) bi-weekly for 10 weeks. Animals were distributed in two groups: partial hepatectomy (n = 6) or partial hepatectomy + scaffold (n = 6). All animals were subject of surgery after sedation-anesthesia with Xylacine-Ketamine. A piece of 1 cm3 was excised from the ventrolateral segment of the liver, in the case of the scaffold group the piece was substituted by the sterile material. 10 days after the surgery, animals received an excess of anesthetics and livers were obtained. The areas of interest were excised. Tissue was collected, fixed and paraffin embedded. 5 μm sections were obtained and stained with Hematoxyline-Eosine or Sirius Red. RESULTS: Surgeries were successfully developed. Rats in the scaffold group did not show any post-surgical alteration. The area of interest in the liver was located by unabsorbable suture stitches. At the macroscopic level, the scaffold grafted was difficult to identify since new tissue had grown on the material. Histological sections revealed the development of new tissue in both groups. The partial hepatectomy + scaffold livers showed cellular growth over the scaffold and no signs of rejection towards the material were observed. CONCLUSIONS: The novel collagen based matrix exhibited biocompatibility with hepatic tissue and was able to allow cellular growth functioning as a scaffold while it was incorporated into the tissue. CONFLICT OF INTEREST: The authors have no relationship to disclose.

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2011-06-13   |   939 visitas   |   Evalua este artículo 0 valoraciones

Vol. 10 Núm.3. Julio-Septiembre 2011 Pags. 376-391 Ann Hepatol 2011; 10(3)